Cell-free DNA in Embryo Culture Media as Non-invasive Biomarker of the Quality of Embryo Cleavage

Background: The success of in vitro-fertilization (IVF) cycles is determined large part by the quality embryo cleavage, which turn, dependent on culture media (CM). Many factors can influence CM, one levels Cell Free Deoxyribonucleic acid (DNA). Understanding association between Cell-free DNA CM and cleavage could help improve IVF techniques.
 Methods: This prospective study was conducted with 96 spent from patients undergoing cycle, order to determine relationships day 3. After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated 3 their development, according cell number. Day 2 corresponding each analyzed, quantitative PCR, for estimation levels.
 Results: results revealed a significant increase 4 6 compared those 7 8 (p=0.04). As showed no difference Cell-Free 7-8 4-6 (p=0.4). Also, free significantly higher both (p=0.03; p=0.04). 
 Conclusion: We conclude that cell-free might be associated delayed cleavage.